Uncubed
           

Mammalian Bioproduction Cell Engineer (CHO, HEK293T)

Ginkgo Bioworks, Boston, MA

THE ORGANISM COMPANY


Our mission is to make biology easier to engineer.  Ginkgo is constructing, editing, and redesigning the living world in order to answer the globe’s growing challenges in health, energy, food, materials, and more.  Our bioengineers make use of an in-house automated foundry for designing and building new organisms.  Today, our foundry is developing over 40 different organisms to make different products across multiple industries.

Ginkgo is continuously crossing new frontiers of biological engineering and we have a record of delivering what was, until recently, thought impossible.  Now, our expansion into mammalian cell engineering promises to push our boundaries even further, and so we’re looking for pioneering engineers to make Ginkgo’s new mammalian cell foundry a success.

We believe there are exciting opportunities to engineer mammalian cells, such as CHO and HEK293T, to consistently higher production titer, more predictable and durable genetic stability, reduced media requirements, and many other valuable parameters.  Ginkgo’s extensive experience in optimizing protein production across a wide range of microbial hosts provides a uniquely strong foundation for the engineering of mammalian bioproduction hosts.  Now is the time to make CHO 2.0 a reality, and we’re looking for highly skilled and creative scientists and engineers to lead our efforts in engineering mammalian bioproduction hosts. 

Responsibilities

  • Identify opportunities and develop strategies for the optimization of protein production in mammalian hosts, such as CHO and HEK293T cells.  Strategies based on existing data and hypotheses in the field should explore a wide range of options from metabolic pathway balancing to secretory pathway optimizations, such as increased chaperone expression. 
  • Utilize Ginkgo’s resources and expertise in metabolomic, proteomic, and sequence profiling to produce comprehensive data sets for metabolic modeling, genetic stability, etc. in order to develop optimization hypotheses for further testing. Take full advantage of capabilities for unbiased interrogation of bioproduction host cells in different production conditions to generate new hypotheses for host cell engineering. 
  • Monitor cell growth, nutrient and metabolite trends, protein concentration, and quality during cell culture at various scales and methods including multiwell plates, shake flasks, wave bags, and bioreactors such as Ambr15 and Ambr250.  
  • Use the data above to propose and test early-stage process parameters for scale-up. Identify, design and implement these key assays for cell production monitoring at appropriate scale and efficiency.
  • Serve as foundry resource for CHO/HEK protein optimization projects, working with teams to design and execute optimization projects, including cell transfection, growth, and analyte testing.

Requirements

  • Hands-on proficiency in mammalian cell culture, especially for CHO and HEK293T cells in a variety of formats (multiwell plates and T flasks required; experience with Ambr15 and Ambr250 bioreactors, wave bags, etc. strongly preferred) 
  • Extensive experience with multiple transfection reagents and protocols, clone selection, and demonstrating clonality

Additional Preferred Requirements (desired, but not essential)

  • Familiarity with laboratory automation and data analysis platforms such as Python, R, and/or JMP. 
  • Hands-on experience with development of workflow automation for biologics
  • Expertise with test assays for protein titer, purity, and quality (SDS-PAGE, LabChip capillary electrophoresis, Western blots, ELISAs, Octet, etc.)
  • Experience with bioproduction and GMP framework
  • Strong knowledge of viral vectors and gene editing techniques
  • Biotech industry experience with CHO and/or HEK293T cell production of recombinant proteins, including antibodies and fusion proteins
To learn more about Ginkgo, check out some recent press:

We also feel it’s important to point out the obvious here – there’s a serious lack of diversity in our industry and it needs to change. Our goal is to help drive that change. We hope to continue to build a company whose culture promotes inclusion and embraces how rewarding it is to work with engineers from all walks of life.  Making biology easier to engineer is a tough nut to crack – we can’t afford to leave any talent untapped.

It is the policy of Ginkgo Bioworks to provide equal employment opportunities to all employees and employment applicants.

About Ginkgo Bioworks

Ginkgo Bioworks is the organism company. We design custom microbes for customers across multiple markets. We build our foundries to scale the process of organism engineering using software and hardware automation. Organism engineers at Ginkgo learn from nature to develop new organisms that replace technology with biology.

Want to learn more about Ginkgo Bioworks? Visit Ginkgo Bioworks's website.